By Md. Rezaul Karim1, Zinat Mahal2, Safia Iqbal1, Harun or Rashid2, M. Abu Hena Mostofa Jamal1, Md. Azizul Islam1, Md. Mafizur Rahman1
- Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh
- Institute of Food and Radiation biology, Atomic Energy Commission, Bangladesh
Lathyrus sativus (Khesari plant) and sugarcane bagasse are considered
as agro wastes. Khesari plants are generally grown in fields as weeds
and they have to be removed prior to cultivation. Taking this view in
mind, we have investigated the conversion of these lignocellulosic
agro-waste as an enriched feed stock for cattle via by solid state
fermentation using a cellulolytic fungus, Pleurotus sajor-caju.
The
strain required 8 weeks to complete the fermentation on both the
untreated and treated (with alkali, lime and presoaked) substrates at
30°C. Higher amounts of reducing sugar and soluble protein were found in
each of the lime treated substrates than untreated substrates. Results
also indicated that presoaked substrate contain higher amounts of
reducing sugar and soluble protein than unsoaked substrate. Among the
substrates, mixed substrate (khesari plant + sugarcane bagasse) was
found to accumulate higher amount of sugar, 22.15 mg/g and protein,
22.80 mg/g than those of khesari plant in the 5th week of fermentation.
The treatments that augmented the level of sugar
and protein were also found to enhance the cellobiase, carboxy methyl cellulase and avicelase activity of crude culture
extracts.These results suggest that lime treatment and presoaking seem to increase the digestibility of the substrates by the fungal cellulolytic enzymes. During eight weeks of
fermentation,relatively higher cellobiase activity was found as compared to that of carboxymethylcellulase and avicelase at 30 o C for
the fungul strain.The results of the present study clearly indicate that fungal conversion with pretreatment transform these lignocellulosic agro-wastes to a nutritionally enriched animal feed.
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