- Pavan J. Kundur1,2,3*, Prakash G. Patil1,4, B.G. Harish2, 3, C.K. Ramesh5, H.E. Shahidhar1
- Department of Plant Biotechnology, University of Agricultural Sciences, Bengaluru -560065, India
- M. S Ramaiah Institute of Technology, Bengaluru- 560054, India
- Visveshwarya Technological University, Belagavi- 590018, India
- Indian Institute of Pulses Research, Kanpur-208024, India
- Sahyadri Science College, Shivamogga – 577203, India
In the present investigation, based on the seven rice putative candidate
iron transporter genes, novel TRAP markers were developed.These
markers were successfully employed in the molecular diversity study
among 30 rice genotypes representing improved rice cultivars and land
races with varied grain iron content (7.38 - 30.58 ppm).
Totally, thirty
TRAP primer combinations were screened, which generated 703 bands out
of which 654 were polymorphic (93%) with an average of 21.8 bands per
primer combination. The average polymorphic information content (PIC)
values ranged from 0.09(Osysl4b+ME05) to 0.25 (Osnramp5c+ME05,
Osnramp1b+ME02 and Osysl4a +ME02). Gene diversity (H ˆ) ranged from 0.10 (Osysl4b+ME05) to 0.31 (Osnramp1b + ME02 and
Osysl4a +ME02). The Jaccard dissimilarity ranged from 0.15 to 0.52,
explaining 37% of genetic variation (Table 4).
Grouping of genotypes
based on UPGMA and principal coordinate analysis (PCoA) were found
comparable and grouping of genotypes into a different cluster was found
mainly on the basis of pedigree relationships. TRAP markers revealed
well resolved relationships among rice genotypes. The information
generated from this study will helps to select parental combinations for
breeding high iron content rice varieties.
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