June 26, 2019

Screening Fenugreek genotypes for high callus induction and growth | IJAAR


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Fenugreek (Trigonella foenume-graecum) is a medicinal herb that has anti-diabetic properties. 4-hydroxyisoleucine, an important metabolite of this plant, is a good candidate for improving hepatic insulin resistance in type 2 diabetic patients.

In vitro production of metabolites mainly relies on the ability of the tissue culture system to produce more and viable cells with maximum production rate. Callus induction is the first step of establishing plant cell based bioreactors which needs potential genotypes with higher and faster callus production. To find such genotypes, screening them is indispensable. In this research, we screened 21 Iranian landraces of fenugreek for callus induction and growth on MS medium supplemented with some plant growth regulators. The results showed different capacity of the screened genotypes in callus induction (P<0.05). Also, the type of explant had a significant effect on callus induction (P<0.05). Calli were able to produce 4-Hydroxyisulosine 67% of the amount in vivo samples. Get the full articles at- Int. J. Agron. Agri. Res. 12(6), 146-154, June 2018.

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International Journal of Agronomy and Agricultural Research - IJAAR is an open-access scholarly research journal, published by International Network for Natural Sciences. IJAAR publishes original scientific research articles in the field of Agronomy and Agricultural Sciences. IJAAR published 2 Volume and 12 issue per the calendar year.

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